Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Experimental Investigation of Phase Inversion of Liquid-Liquid Systems in a Spray Extraction Column

An experimental study of the phase inversion behavior of liquid-liquid dispersion has been conducted in a spray extraction column for systems of toluene / water, n-hexane/water, CCl4/water, toluene /water + glycerol (25 % wt), toluene + CCl4 (25 % wt) / water and toluene / acetic acid (5 % wt)/water. The effects of physical properties, mass transfer and column geometry...

Liquid-Liquid Two-Phase Flow Systems

experimental investigation of phase inversion of liquid-liquid systems in a spray extraction column

an experimental study of the phase inversion behavior of liquid-liquid dispersion has been conducted in a spray extraction column for systems of toluene / water, n-hexane/water, ccl4/water, toluene /water + glycerol (25 % wt), toluene + ccl4 (25 % wt) / water and toluene / acetic acid (5 % wt)/water. the effects of physical properties, mass transfer and column geometry on phase inversion have b...

Extraction and separation of proteins by ionic liquid aqueous two-phase system.

A satisfactory protocol of protein extraction and separation has been established based on the ionic liquid aqueous two-phase system (IL-ATPS) for the purification of bioactive substances. Compared with the effects of eight different ionic liquids, 1-octyl-3-methylimidazolium bromide ([omim][Br]) was selected as the suitable ionic liquid. Based on the single-factor experiment, an initial serial...

Extraction and Process Conditions Optimization of Ficin by Ionic Liquid Aqueous Two-phase System

In order to research the application of [C4mim] Br-K2HPO4 ionic liquid aqueous two-phase system to extract ficin, the influence of reagents to the activity of ficin have been investigated. This study aimed to investigate the effects of species and concentration of the ionic liquid, the dosage of ficin, pH value and temperature on the partitioning behavior of ficin, respectively. Response surfac...